IMPACT OF LACTOBACILLUS PLANTARUM (NCDC LP20) PROBIOTIC BACTERIA ON ENHANCED INNATE IMMUNO PARAMETERS IN LABEO ROHITA (ROHU)

  • S. Janardana Reddy Department of Fishery Science and Aquaculture, College of Sciences, Sri Venkateswara University, Tirupati, Andhra Pradesh, India
  • Subhan Ali Department of Biochemistry, College of Sciences, Sri Venkateswara University, Tirupati, Andhra Pradesh, India
DOI: https://doi.org/10.53555/ar.v4i10.2482
Keywords: Aeromonas hydrophila, Differential leukocyte counts, Labeo rohita, Lactobacillus plantarum (NCDC LP20), Respiratory burst activity, Serum bactericidal activity

Abstract

In according to several reports, the advantageous effects of bacteria on fish have well been documented. Probiotics as an alternative strategy have been suggested to be used as reinstatement for antimicrobial drugs and growth promoters. Some researchers believe that Probiotics have an advantageous for strengthening the health of fish in aquaculture and augmenting fish performance. The present study was designed to investigate the enhanced effects in innate immunological indices of by the oral administration of probiotic Lactobacillus plantarum (NCDC LP20) in freshwater fish, Labeo rohita (Rohu). Probiotic was administered orally at three different doses 1×106 (T2), 1.5×106 (T3), 2×106 (T4) cfu/g feed to Labeo rohita for four weeks. The positive control group (T1) and negative control group (T5) was fed feed without probiotic for the same period. On 29thday, blood and serum samples were collected to determine differential leukocyte counts (DLC), respiratory burst activity (NBT assay) and serum bactericidal activity. Fishes were challenged intra peritoneal injection with  Aeromonas hydrophila after four weeks in the treatment groups (T2, T3 and T4) and also in the positive control group (T1), while the negative control group (T5) was administered with phosphate buffer saline (PBS pH 7.2). The NBT assay and DLC were assessed on 7th day post challenge. Probiotic Lactobacillus plantarum (NCDC LP20) treated fish showed significantly higher (P <0.05) respiratory burst activity and bactericidal activity during the pre-challenge compared with the control groups. The highest respiratory burst activity (0.42±0.01) and serum bactericidal activity was recorded in the group (T4) fed feed containing Lactobacillus plantarum (NCDC LP20) at 2×106 cfu/g feed. Granulocytes numbers were significantly higher (P <0.05) in treatment groups in comparison to the control in both the pre and post challenge periods. The result suggests that Lactobacillus plantarum (NCDC LP20) significantly enhance innate immune response in Labeo rohita (Rohu).

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References

Al-Dohail, M. A., Hashim, R., & Aliya-paiko, M. (2009). Effects of the probiotics, Lactobacillus acidiphilus, on the growth performance, hematology parameters and immunoglobulin concentration in African catfish fingerling. Aquaculture Research, 40, 1642-1652.
Abraham, TJ, Banerjee, T. Beneficial antagonistic bacteria from freshwater and culture environment as probiotics in ornamental fish culture, Indian J. Fish. 54(3), 2007, 311-319.
Aly, SM, Ahdmed, YA, Ghareeb, AA, Mohamed, MF. Studies on Bacillus subtilisand Lactobacillus acidophilus, as potential probiotics, on immune response of Tilapia nilotica (Oreochromis niloticus) to challenge infections, Fish Shellfish Immunol., 25, 2008,128-136.
Austin, B, Stuckey, LF, Robertson, PAW, Effendi, I, Griffith, DRW. A probiotic strain of Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii. J. Fish Dis. 18, 1995, 93-96.
Barefoot, SF, Klaenhammer, TR. Detection and activity of Lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl. Environ. Microbiol. 45, 1983, 1808-1815.
Cabello, F.C. (2006). Heavy use of prophylactic antibiotics in aquaculture: A growing problem for human and animal health and for the environment. Environ. Microbiol. 8, 1137–1144.
Carnevali, O, Zamponi, MC, Sulpizio, R, Rollo, A, Nardi, M, Orpianesi, C, Silvi, S, Caggiono, M, Polzonetti, AM, and Cresci, A. Administration of probiotic strain to improve sea bream well ness during development. Aquacult. Int. 12, 2004, 377-386.
Contessie B, Volpatti D, Galeotti M. Evaluation of immunological parameters in farmed gilthead sea bream, Sparus aurata L., before and during outbreaks of winter syndrome. Journal of Fish Diseases 2006; 29:683-690.
Dawood,M.A.O. Koshio, S. (2016). Recent advances in the role of probiotics and prebiotics in carp aquaculture: A review. Aquaculture. 454, 243–251.
De-la-Banda, IG, Chereguini, O, Rasines, I. Influence of lactic acid bacterial additives on turbot (Scopthalmus maximus L.) larvae culture. Bol. Inst. Esp. Ocean org. 8, 1992, 247-254.
Douillet, PA, Langdon, CJ. Use of a probiotic for the culture of larvae of the Pacific oyster (Crassostreagigas thunberg). Aquaculture 119, 1994, 25-40.
Gatesoupe, FJ. Lactic acid bacteria increase the resistance of turbot larvae, Scophthalmus maximus, against pathogenic Vibrio. Aquat. Living Res. 7, 1994, 277-282.
Gatesoupe, FJ. Siderophore production and probiotic effect of Vibrio sp. Associated with turbot larvae, Scopthalmus maximus. Aquat. Living Res., 7, 1991b, 277-282.
Gildberg, A, Johansen, A, Bogwald, J. Growth and survival of Atlantic salmon (Salmo salar) fry given diets supplemented with fish protein hydrolysate and lactic acid bacteria during a challenge trial with Aeromonas salmonicida. Aquaculture 138, 1995, 23–34.
Gildberg, A, Mikkelsen, H, Sandaker, E, Ringo, E. Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of Atlantic cod (Gadus morhua). Hydrobiologia 352, 1997, 279—285.
Guerreiro, I. Couto, A. Machado, M. Castro, C. Pousao-Ferreira, P. Oliva-Teles, A. Enes, P. (2016). Prebiotics effect on immune and hepatic oxidative status and gut morphology of white sea bream (Diplodus sargus). Fish Shellfish Immunol. 50, 168–174.
Holzapfel, WH, Haberer, P, Snel, J, Schillinger, U, Huisin't Veld J. Overview of gut flora and probiotics. Int. 1. Food Microbiol. 41, 1998, 85-101.
Kim DH, Austin B. Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish and Shellfish Immunology 2006; 21:513-524.
Martínez Cruz, P. Ibanez, A.L. Monroy Hermosillo, O.A. Ramirez Saad, H.C.(2012). Use of Probiotics in Aquaculture.ISRN Microbiol. 2012, 1–14.
Kumar, G, Sharma, R. Probiotics-the mainstay in aquaculture health management. Info fish Int. 5, 2001, 42-47.
Nikoskelainen S, Ouwehand AC, Bylund G, Salminen S, Lilius EM. Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish and Shellfish Immunology 2003; 15:443-452.
Olafsen, JA. Interactions between hosts and bacteria in aquaculture, In: Proceedings from the US-EC Workshop on Marine Microorganisms: Research Issues for Biotechnology. European Commission, Brussels, Belgium, pp. 1998, 127-145.
Raa, J. The use of immuno-stimulatory substances in fish and shellfish farming. Rev. Fish. Sci. 4, 1996, 229–288.
Rainger GE, Rowley AF. Antibacterial activity in the serum and mucus of rainbow trout, Oncorhynchus mykiss, following immunization with Aeromonas salmonicida. Fish and Shellfish Immunology 1993; 3:475-482.
Rajesh Kumar, Mukherjee SC, Ritesh R, Nayak SK. Enhanced innate immune parameters in Labeo rohita (Ham.) following oral administration of Bacillus subtilis. Fish & Shellfish Immunology 24, 2008, 168-172.
Rengpipat, S, Rukpratanporn, S, Piyatiratitivorakul, S, Menasaveta, P. Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S 11). Aquaculture 191, 2000, 271-288.
Ringo, E, Gatesoupe, FJ. Lactic acid bacteria in fish. A review. Aquaculture 160, 1998, 177–203.
Sahoo, T.K. Jena, P.K. Nagar, N. Patel, A.K. Seshadri, S. (2015). In vitro evaluation of probiotic properties of lactic acid bacteria from the gut of Labeo tohita and Catla catla. Probiotics Antimicrob. Proteins 2015, 7, 126–136.
Sakai, M. Current research status of fish immunostimulants. Aquaculture 172, 1999, 63-92.
Stasiack AS, Baumann CP. Neutrophil activity is a potent indicator of concomitant analysis. Fish and Shellfish Immunology, 1996, 6:53-79.
Strom, E, Ringo, E. Changes in the bacterial composition of early developing cod, Gadus morhua(L.), larvae following inoculation of Lactobacillus plantarum into the water. In:Walther, 8, Fyhn, H.J. (Eds.), Physiological and Biochemical Aspects of Fish Development. University of Bergen, Norway, pp. 1993, 226-228.
Sung, HH, Kou, GH, Song, and YL. Vibriosis resistance induced by glucan treatment in tiger shrimp (Penaeusmonodon). Fish Pathol. 29, 1994, 11-17.
Sung, HH, Yang,YL, Song,YL. Enhancement of microbiocidal activity in the tiger shrimp (Penaeus monodon) via inmunostinulation. J. Crustacean BioI. 16, 1996, 278-284.
Zacconi, C, Paladino, M, Sarra, PG., Bottazzi, V. Bacteriocin sproduites par Lactobacillus receteri. Microbiologic Aliments Nutr. 3, 1985, 153-156.
Published
2018-10-31
How to Cite
Reddy, S. J., & Ali, S. (2018). IMPACT OF LACTOBACILLUS PLANTARUM (NCDC LP20) PROBIOTIC BACTERIA ON ENHANCED INNATE IMMUNO PARAMETERS IN LABEO ROHITA (ROHU). IJRDO-Journal of Agriculture and Research (ISSN: 2455-7668), 4(10), 07-17. https://doi.org/10.53555/ar.v4i10.2482
Issue
Vol. 4 No. 10 (2018): IJRDO - Journal of Agriculture and Research | ISSN: 2455-7668
Section
Articles

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